第 43 卷第 1 期Vol. 43 No. 1
2013 年 2 月Feb 2013
所属栏目：分析与测试

化学计量学二阶校正法结合三维荧光光谱技术同时快速测定人血清样中西酞普兰、氟西汀和帕罗西汀含量
张喜华，吴海龙*，康 超，王 予，俞汝勤 （湖南大学 化学生物传感与计量学国家重点实验室，湖南 长沙 410082）
摘　要：应用化学计量学二阶校正方法结合三维荧光光谱技术可以实现人血清样中西酞普兰、氟西汀和帕罗西汀含量的直接、快速同时测定。设定荧光激发波长范围为210~260 nm，发射波长为270~388 nm，测定不同样本的三维荧光光谱，构建三维响应数阵。当选取体系组分数为4时，采用PARAFAC方法得到的血清样中西酞普兰、氟西汀和帕罗西汀的加标回收率（95%置信区间）分别为95.9±3.22%、104.5±8.17%和96.4±5.15%，并采用品质因子和椭圆置信区间测试对结果进行了进一步验证。实验结果表明，该方法快速简便，解析结果可靠，无需样本预处理，能直接用于同时分析人血清中西酞普兰、氟西汀和帕罗西汀的含量。
关键词：三维荧光光谱；二阶校正；西酞普兰；氟西汀；帕罗西汀
中图分类号：O6571　　文献标识码：A　　文章编号：1009-9212（2013）01-0063-06
Simultaneous and Rapid Determination of Citalopram，Fluoxetine and Paroxetine Contents in Human Serum Samples Using Three-dimensional Fluorescence Technique Coupled with Chemometric Second-order Calibration
ZHANG Xi-hua，WU Hai-long*，KANG Chao，WANG Yu，YU Ru-qin （State Key Laboratory of Chemo／Biosensing and Chemometrics，Hunan University，Changsha 410082，China）
Abstract：A novel and effective approach was developed for the simultaneous determination of citalopram（CIT），fluoxetine（FLU）and paroxetine（PAR）in human plasma samples by the incorporation of excitation-emission fluorescence（EEM）and second-order calibration method based on the parallel factor analysis（PARAFAC）algorithm. The scanning range of their excitation and emission wavelengths were set at 210～260 nm and 270～380 nm，respectively. The obtained 95% confidence interval of CIT，FLU and PAR in human serum samples by using PARAFAC method with an estimated component number of four were 95.9±3.22，104.5±8.17 and 96.4±5.15%，respectively. Moreover，the accuracy of the method was also evaluated through elliptical joint confidence region（EJCR）tests as well as the figures of merit. The experimental results demonstrated that the method has been satisfactorily applied to determination of CIT，FLU and PAR in human serum samples without previous separation procedure.
Key words：three-dimensional fluorescence spectroscopy；second-order calibration；citalopram；fluoxetine；paroxetine
基金项目：国家自然基金资助项目（21175041）。
作者简介：张喜华（1986-），女，湖南湘潭人，硕士研究生，从事化学计量学多维校正方法的应用研究。（E-mail：zhangxihua0720@163.com）
联 系 人：吴海龙，教授，主要从事化学计量学、化学生物传感技术研究。（E-mail：hlwu@hnu.edu.cn）
收稿日期：2013-02-25