第 44 卷第 3 期 Vol. 44 No. 3 2014 年 6 月 Jun 2014

所属栏目：分析与测试 高效液相色谱-荧光法测定阿维菌素在稻田环境基质中的残留

宁 赢1，彭秧锡1，欧晓明1，2，3*，梁 骥2，3，张贵群2，3，刘义珂2，3，聂思桥2，3，任 竞2，3，刘金胜2，3，傅 强2，3 （1. 湖南人文科技学院，湖南 娄底 417000；2. 湖南化工研究院 国家农药创制工程技术研究中心，湖南 长沙 410014；3. 农用化学品湖南省重点实验室，湖南 长沙 410014）

摘　要：采用高效液相色谱-荧光检测器（HPLC-FLD）建立了阿维菌素在水稻植株、谷壳、糙米、土壤及田水中的残留检测方法。样品用乙腈提取，经液液萃取净化，浓缩定容后衍生化，HPLC-FLD检测，外标法定量。结果表明，在添加水平为0.01～0.50 mg／kg时，阿维菌素在水稻植株、谷壳、糙米、土壤和田水中的平均添加回收率为79.13%～102.98%，相对标准偏差为3.10%～6.71%。该方法的最小检出量为0.2 ng，在水稻植株、土壤、谷壳、糙米和田水中的最低检测浓度均为0.01 mg／kg。

关键词：高效液相色谱-荧光检测器；阿维菌素；稻田；残留

中图分类号：O657.7+1　　文献标识码：A　　文章编号：1009-9212（2014）03-0066-04

Determination of Abamectin Residues in Paddy Field Environmental Matrices by High Performance Liquid Chromatography with Fluorescence Detector

NING Ying1, PENG Yang-xi1, OU Xiao-ming1,2,3*, LIANG Ji2,3, ZHANG Gui-qun2,3, LIU Yi-ke2,3, NIE Si-qiao2,3, REN Jing2,3, LIU Jin-sheng2,3, FU Qiang2,3 (1. Hunan University of Humanities, Science and Technology, Loudi 417000, China; 2. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry, Changsha 410014, China; 3. Hunan Province Key Laboratory for Agrochemicals, Changsha 410014, China)

Abstract：A method for the determination of abamectin residue in paddy plants, husk, coarse rice, soil and water was established using high performance liquid chromatography with fluorescence detector(HPLC-FLD). The samples were extracted with acetonitrile, cleaned up by liquid-liquid separation, and directly pre-column derivatized after concentration and constant volume. The analysis was performed by HPLC-FLD with an external standard. The results showed that the average recoveries of abamectin in rice plants, husk, coarse rice, soil and water were from 79.10% to 102.98%, and the relative standard deviations were from 3.10% to 6.71% when the spiked levels ranged from 0.01 mg/kg to 0.50 mg/kg. The LOD was 0.2 ng, and the LOQ was 0.01 mg/kg in paddy plants, husk, rice, soil and water.

Key words：HPLC-FLD; abamectin; paddy field; residues

作者简介：宁 赢（1990-），男，湖南衡阳人，硕士研究生，研究方向：农药残留检测（E-mail：1005861004@qq.com）。

联 系 人： 欧晓明，研究员，博士，研究方向：农药残留化学、环境毒理和农药生物学（E-mail：xmouhn@163.com）。

收稿日期：2014-05-16