第 44 卷第 5 期 Vol. 44 No. 5 2014 年 10 月 Oct 2014

所属栏目：分析与测试 分散液液微萃取-气相色谱分析尿碘含量

叶海朋，江 阳，周 凯 （杭州市上城区疾病预防控制中心，浙江 杭州 310009）

摘　要：以过硫酸铵消解处理尿液，用丁酮衍生后以二硫化碳为微萃取剂，甲醇为分散剂，用分散液液微萃取技术提取尿中碘代丁酮，以气相色谱-电子捕获检测器分析，用外标法定量。尿碘的线性范围为10~300 μg／L，相关系数r=0.999 5，检出限0.20 μg／L；方法回收率达91.9%~97.7%，相对标准偏差为2.4%~z4.6%（n=6）。该方法操作简便、灵敏度高，适于尿碘批量快速分析。

关键词：尿碘；分散液液微萃取；气相色谱

中图分类号：O657.7+1　　文献标识码：A　　文章编号：1009-9212（2014）05-0070-03

Analysis of Iodine in Urine by Dispersive Liquid-liquid Micro-extraction and Gas Chromatography

YE Hai-peng, JIANG Yang, ZHOU Kai (Shangcheng District Center for Disease Control and Prevention, Hangzhou 310009, China)

Abstract：To detect iodine in urine by dispersive liquid- liquid micro-extraction and gas chromatography, the urine was pre-treated with ammonium persulfate and 2-butanone, and then extracted with carbon disulfide and methanol. Iodobutanone in the extract was analyzed with GC- μECD and quantified by means of external standard curve. The detection limit of the iodine in urine was 0.20 μg/L, and the standard curve was linear in the range of 10~300 μg/L (r=0.9995). The average recovery rate of iodine was 91.9% ~97.7% and the relative standard deviation (RSD) was from 2.4% to 4.6% (n=6). Conclusion: This method is simple, highly sensitive and suitable for quick analysis of iodine in urine.

Key words：iodine urine; dispersive liquid-liquid micro-extraction; gas chromatography

基金项目：浙江省医药卫生科技计划项目（2011KYB087）。

作者简介： 叶海朋（1986-），男，安徽安庆人，检验技师，从事卫生检验工作。

收稿日期：2014-09-25