第 45 卷第 2 期Vol. 45 No. 2
2015 年 4 月Apr 2015
所属栏目：分析与测试

HPLC法测定N-羟基-N-（2-（（1-（4-氯苯基）-1H-吡唑-3-基）氧）甲基）苯基氨基甲酸甲酯
孔晓红1，2，王艳丽1，2 （1. 湖南化工研究院 国家农药创制工程技术研究中心，湖南 长沙 410014；2. 农用化学品湖南省重点实验室，湖南 长沙 410014）
摘　要：采用Aglient Extend-C18 5 μm 250 mm×4.6 mm色谱柱，流动相为甲醇-0.4%磷酸溶液（v∶v=75∶25），流速为1 mL／min，275 nm为检测波长，建立了测定N-羟基-N-（2-（（1-（4-氯苯基）-1H-吡唑-3-基）氧）甲基）苯基氨基甲酸甲酯含量的高效液相色谱分析方法。在进样量为0.53～3.52 μg的范围内线性关系良好（y=125679.4x+15010.4，r=0.9999），RSD 0.24%，回收率99.2%～100.4%，分析周期为15 min。方法快速、简便、准确，可用于N-羟基-N-（2-（（1-（4-氯苯基）-1H-吡唑-3-基）氧）甲基）苯基氨基甲酸甲酯反应液及产品的质量控制
关键词：N-羟基-N-（2-（（1-（4-氯苯基）-1H-吡唑-3-基）氧）甲基）苯基氨基甲酸甲酯；HPLC；测定
中图分类号：O657.7+2　　文献标识码：A　　文章编号：1009-9212（2015）02-0070-03
Determination of methyl 2-((1-(4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate by HPLC
KONG Xiao-hong1, WANG Yan-li1 (1. Hunan Research Institute of Chemical Industry, National Engineering Research Center for Agrochemicals, Changsha 410014, China; 2. Hunan Province Key Laboratory for Agrochemicals, Changsha 410014, China)
Abstract：A HPLC method was established for the determination of methyl 2-((1-(4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate. The HPLC system consisted of Aglient Exlend-C18 column(5 μm, 4.6 mm×150 mm), a mixture of methanol and 0.4% phosphoric acid aquas solution at a volume-ratio of 75 to 25 as mobile phase, 275 nm as the detected wavelength, a flow rate of 1mL/min and column temperature of 40℃. The experimental results showed that this method for the determination of methyl 2-((1-(4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate had a good linear correlation in a rang from 0. 53 μg to 3.52 μg(y=1 256 794.4x+15 010.4; r=0.999 9). RSD was 0.24% and the recovery was in a rang from 99.2% to 100.4%. The complete period of analysis was 15 min. This method was proved to be sensitive, fast, and accurate. It could be applied in the quality control of methyl 2-((1-(4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate.
Key words：methyl 2-((1-（4-chlorophenyl)-1H-pyrazol-3-yloxy)methyl)phenyl(hydroxy)carbamate
作者简介：孔晓红（1972-），女，湖南长沙人，助理研究员，主要从事农药、精细化工中间体分析（E-mail：hehuayuan4602@sina.com）。
收稿日期：2015-01-10