第 46 卷第 3 期 Vol. 46 No. 3 2016 年 6 月 Jun 2016

所属栏目：医药及中间体 乙醛对假丝酵母脂肪酶催化活性的影响

魏英民，熊龙斌，付薪菱，徐 超，周建波* （长沙医学院，湖南 长沙 410219）

摘　要：采用紫外可见分光光度法和荧光发射光谱法研究了乙醛溶液对假丝酵母脂肪酶水解三油酸甘油酯的催化活性和构象的影响。结果表明，低浓度乙醛能提高酶的催化水解活力，当乙醛浓度为0.221 5 mmol／L时，酶活力提高了18.84%；高浓度乙醛对酶活力有抑制作用，当乙醛浓度为3.322 5 mmol／L时，酶活力降低了25.85%。低浓度乙醛使酶催化反应的最适pH向碱性方向偏移，紫外吸收光谱和荧光发射光谱均有显著增强和光谱峰偏移现象。动力学分析表明，加入乙醛后，酶的Vmax增大，Km减小。

关键词：乙醛；假丝酵母脂肪酶；酶活性；光谱分析

中图分类号：TQ921　　文献标识码：A　　文章编号：1009-9212（2016）03-0028-04

Effect of Acetaldehyde on Catalytic Activity of Candida Rugosa Lipase

WEI Ying-min, XIONG Long-bin, FU Xin-ling, XU Chao, ZHOU Jian-bo* (Changsha Medical University, Changsha 410219, China)

Abstract：The alteration of Candida rugosa lipase(CRL) activity and conformation were studied after pretreatment with acetaldehyde solutions at different concentrations by UV-vis spectroscopy and fluorescence emission spectroscopy. The results showed that the lipase activity increased in the presence of acetaldehyde at low concentration and the highest increasement was 18.84% when the acetaldehyde concentration was 0.221 5 mmol·L-1. The lipase activity decreased in the presence of acetaldehyde at high concentration and the highest decreasement was 25.85% when the acetaldehyde concentration was 3.322 5 mmol·L-1. The optimum pH value of the 1ipase in the presence of acetaldehyde increased by 0.5 compared to that in the absence of acetaldehyde. The UV absorption and fluorescence emission intensities of the lipase in the presence of acetaldehyde were enhanced markedly and the spectral peaks shifted conpared to the contrast. When CRL was treated with acetaldehyde, the Vmax increased while Km value declined．

Key words：acetaldehyde; Candida rugosa lipase; enzyme activity; spectrum analysis

基金项目：湖南省教育厅项目（14C0121和13C1127）。

作者简介：魏英民（1985-），女，山东聊城人，硕士研究生，研究方向：酶催化（E-mail：weiym929@163.com）。

联 系 人：周建波，女，湖南衡阳人，讲师，研究方向：有机催化（E-mail：Zhoujianbo0603@126.com）。

收稿日期：016-05-24