第 47 卷第 3 期 Vol. 47 No. 3 2017 年 6 月 Jun 2017

所属栏目：染料、颜料及涂料 Ni2+近红外荧光分子探针的制备与应用研究

蒋银燕，罗春花，王翠琼，张青芳，郭丽娟，左雅婵* （长沙医学院，湖南 长沙 410219）

摘　要：合成了近红外荧光探针Cyclam-IR-780，该探针的最大激发波长为532 nm，最大发射波长为610 nm，stokes位移明显增大，达78 nm，可避免对生物活体细胞的损伤。探针与Ni2+结合后形成的配位化合物荧光强度变化明显。考察了pH值及其它金属离子对Ni2+检测的影响，结果表明，在pH=7.40的生理环境下，该探针对生物体内常见的金属离子干扰较小。Ni2+的检测下限为1.74×10-4 mmol·L-1，显示了较高的检测灵敏度。

关键词：近红外荧光分子探针；Ni2+；Cyclam-IR-780

中图分类号：O657　　文献标识码：A　　文章编号：1009-9212（2017）03-0043-04

The Preparation and Application of a Near-infrared Fluorescent Molecular Probe for Ni2+

JIANG Yin-yan, LUO Chun-hua, WANG Cui-qiong, ZHANG Qing-fang, GUO Li-juan, ZUO Ya-chan* (Changsha Medicial University, Changsha 410219, China)

Abstract：The development of novel near-infrared fluorescent probe for Ni2+, Cyclam-IR-780， was described in this paper, which based on cyclam as the recognition group to a near-infrared fluorophore IR-780. The excitation and emission wavelengths of the Cyclam-IR-780 were 532 nm and 610 nm respectively, and its fluorescence intensity increased with the addition of Ni2+ under physiological condition(pH=7.40) and the detection limit reached 1.74×10-4 mmol·L-1. Moreover, the fluorescent intensity of Cyclam-IR-780 did not increase noticeably in the presence of other biologically important cations. The results showed that the probe was not only able to effectively minimize cell damage and autofluorescence by excitation light but also afford sufficient sensitivity and selectivity for the biological detection of Ni2+.

Key words：near-infrared fluorescent molecular probe; Ni2+; Cyclam-IR-780

基金项目：湖南省教育厅科学研究项目（16C0153，17C0151），湖南省高校教学改革研究项目（湘教通〔2016〕400号）。

作者简介：蒋银燕（1981-），女，湖南益阳人，讲师，硕士研究生，研究方向：生化分离分析（E-mail：jyyhello@126.com）。

联 系 人：左雅婵，讲师，研究方向：临床医学检验（E-mail：442968439@qq.com）。

收稿日期：2017-06-15