第 49 卷第 2 期Vol. 49 No. 2
2019 年 4 月Apr 2019
所属栏目：香精香料

超临界CO2法提取丁香、迷迭香复方精油及其活性研究
周君健，谭 湛，李燕晖，罗金花* （长沙学院 生物与环境工程学院，湖南 长沙 410022）
摘　要：采用超临界CO2萃取法提取不同原料质量比例丁香和迷迭香复方精油，气质联用仪（GC-MS）对其进行化学成分分析，并通过DPPH法对其抗氧化活性进行测定，采用滤纸片抑菌圈法对其进行抑菌活性研究。结果表明，复方精油原料丁香含量越高，其抗氧化活性越好；而复方精油［m（原料丁香）∶m（迷迭香）=1∶3］抑菌活性最好。在丁香精油中鉴定出19种物质，其主要成分为丁香酚和乙酸丁香酚酯；在迷迭香精油中鉴定出62种物质，其主要成分为桉叶油醇、樟脑等；在活性最佳复方精油中，共鉴定出19种物质，其主要成分为桉叶油醇、樟脑、丁香酚、乙酸丁香酚酯等。
关键词：复方精油；超临界CO2萃取法；抗氧化活性；抑菌活性；GC-M
中图分类号：TQ655　　文献标识码：A　　文章编号：1009-9212（2019）02-0058-08
Extraction and Activity of Syzygium Aromaticum and Rosmarinus Officinalis Compound Essential Oil by Supercritical CO2
ZHOU Jun-jian, TAN Zhan, LI Yan-hui, LUO Jin-Hua* (School of Biological and Environmental Engineering, Changsha University, Changsha 410022, China)
Abstract：Syzygium aromaticum and rosmarinus officinalis essential oils were obtained from different ratio of raw materials by supercritical CO2 extraction. Their chemical components were analyzed by GC-MS. Their antioxidant activities were determined by DPPH method, and their antimicrobial activities were studied by filter paper bacteriostasis circle method. The results showed that the higher the content of syzygium aromaticum in raw materialwas, the better its antioxidant activity of compound essential oil was, and the best antimicrobial activity of compound essential oil (mass ratio of raw materials syzygium aromaticum to rosmarinus officinalis M=1∶3). 19 substances were identified in syzygium aromaticum essential oil, including eugenol and eugenol acetate, 62 substances were identified in rosmarinus officinalis essential oil, including eucalyptol and camphor, and 19 substances were identified in compound essential oil with the best activity, including eucalyptol, camphor, eugenol and eugenol acetate.
Key words：compound essential oil; supercritical CO2 extraction; antioxidant activity; bacteriostasis activity; GC-MS
基金项目：湖南省自然科学基金青年项目（2017JJ3340），湖南省教育厅一般项目（17C0127），长沙市科技局一般项目（k1705047），长沙学院博士科研启动基金项目（SF1603），长沙学院2018年大学生科技创新项目计划资助。
作者简介：周君健（1999-），男，湖南长沙人，研究方向：植物精油综合性能的研究（E-mail：3483177394@qq.com）。
联 系 人：罗金花，讲师，博士，主要从事理论计算及天然产物研究（E-mail：jhluo@ccsu.cn）。
收稿日期： 2019-04-1