| 第 49 卷第 3 期 |  | Vol. 49 No. 3 | | 2019 年 6 月 | Jun 2019 |
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所属栏目：医药及中间体
连续液-液萃取法制备高含量液体姜辣素 |
| 凌 琳，李英奇，戴世杰，曾健青*
（湖南和广生物科技有限公司，湖南 永州 425000） |
| 摘 要：以超临界二氧化碳萃取所得的姜树脂为原料，采用连续液-液萃取法进行分离提纯，高效制备高含量液体姜辣素。考察了萃取时间、溶剂与溶质质量比、萃取溶剂流量、萃取温度等对产物姜辣素、6-姜酚含量及收率的影响。结果表明，以80%乙醇水溶液为溶剂，姜树脂为溶质，溶剂与溶质的质量比为6∶1；正己烷为萃取溶剂，萃取溶剂流量为1.0 L·h-1，萃取温度为30℃，萃取时间为3 h，此条件下萃取结果最好，可将姜辣素含量从50.29%提高至83.59%，对应6-姜酚的含量从34.77%提高到68.62%，姜辣素和6-姜酚的收率分别达90.96%和86.01%。 |
| 关键词：姜辣素；6-姜酚；连续液-液萃取法；分离提纯；姜树脂 |
| 中图分类号：R284.1 文献标识码：A 文章编号：1009-9212（2019）03-0016-05 |
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| Preparation of High Content Liquid Gingerol by Continuous Liquid-liquid Extraction |
| LING Lin, LI Ying-qi, DAI Shi-jie, ZENG Jian-qing*
(Hunan Health-Guard Biotechnology Incorporated Company, Yongzhou 425000, China) |
| Abstract：Using ginger oleoresin extracted from ginger by supercritical carbon dioxide extraction method as the raw material, high content liquid gingerol was prepared efficiently through separation and purification by continuous liquid-liquid extraction. The effects of factors including extraction time, solvent mass ratio, extraction solvent flow rate and extraction temperature on the content and yield of gingerol were evaluated. The optimum conditions were obtained as followings: 80% ethanol solution was used as solvent; ginger oleoresin was used as solute; the mass ratio of solvent to solute was of 6; the extraction solvent flow rate was of 1.0 L·h-1 using nhexane as extraction solvent; extraction temperature was of 30℃; extraction time was of 3 h. Under these process parameters, the content of gingerol could be increased from 50.29% to 83.59%; meanwhile the content of 6-gingerol could be increased from 34.77% to 68.62%; the yield of gingerol and 6-gingerol could reach up to 90.96% and 86.01%, respectively. |
| Key words：gingerol; 6-gingerol; continuous liquid-liquid extraction; separation and purification; ginger oleoresin |
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基金项目： 湖南省创新创业技术投资项目（2017GK5038），湖南省企业科技创新创业团队项目（和广生物现代萃取分离与应用科技创新创业团队）。
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作者简介：凌 琳（1991-），女，湖南株洲人，硕士，研究方向：植物提取、分离纯化与应用（E-mail：18874848142@163.com）。
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联 系 人：曾健青，研究员，博士，研究方向：超临界流体技术研究与应用（E-mail：jackingzeng@vip.163.com）。
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收稿日期： 2019-04-0
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