第 49 卷第 4 期 Vol. 49 No. 4 2019 年 8 月 Aug 2019

所属栏目：医药及中间体 6-姜酚的制备与结构表征

凌 琳，李英奇，邓珊霞，曾健青* （湖南和广生物科技有限公司，湖南 永州 425000）

摘　要：以超临界二氧化碳萃取所得的姜树脂为原料，采用连续液-液萃取法制得6-姜酚粗品，再经过加压硅胶柱层析法，对其进行分离纯化，得到含量为95%以上的6-姜酚，通过质谱与核磁共振波谱对其进行结构表征。考察原料中6-姜酚含量、硅胶目数、操作温度、洗脱溶剂流速等对6-姜酚柱层析分离效果的影响。结果表明，在层析硅胶目数为200~300目、操作温度为10℃、洗脱溶剂的流速为1.35 mL·cm-2·min-1、6-姜酚粗品中6-姜酚含量为64.32%的条件下，进行加压柱层析，制备得到含量为95.12%的6-姜酚，6-姜酚的回收率81.46%。

关键词：6-姜酚；姜树脂；加压硅胶柱层析法；结构表征

中图分类号：R284　　文献标识码：A　　文章编号：1009-9212（2019）04-0035-06

Preparation and Structural Characterization of 6-Gingerol

LING Lin, LI Ying-qi, DENG Shan-xia, ZENG Jian-qing* (Hunan Health-Guard Biotechnology Incorporated Company, Yongzhou 425000, China)

Abstract：Using ginger oleoresin as the raw materia extracted from ginger by supercritical carbon dioxide extraction method, 6-gingerol with a purity of more than 95% was prepared efficiently through separation and purification by continuous liquid-liquid extraction and pressurized silica gel column chromatography. The obtained 6-gingerol was characterized by mass spectrometry and nuclear magnetic resonance spectroscopy. The effects of factors including the content of 6-gingerol in raw materials, the size of silica gels, operating temperature and the flow rate of elute on the separation efficiency of 6-gingerol were evaluated. The results showed that 6-gingerolwith a purity of 95.12% could be obtained rapidly with a yield of 81.47% under the following conditions: that the purity of 6-gingerol as raw material for pressurized silica gel column chromatography was of 64.32%; the size of silica gel was of 200~300 mesh; operating temperature was of 10℃ and the elute flow rate was of 1.35 mL·cm-2·min-1 for a gradient elution.

Key words：6-gingerol; ginger oleoresin; pressurized silica gel column chromatography; structural characterization

基金项目：湖南省创新创业技术投资项目（2017GK5038），湖南省企业科技创新创业团队项目（和广生物现代萃取分离与应用科技创新创业团队）。

作者简介：凌 琳（1991-），女，湖南株洲人，硕士，研究方向：植物提取、分离纯化与应用（E-mail：18874848142@163.com）。

联 系 人：曾健青，博士，研究员，研究方向：超临界流体技术研究与应用（E-mail：jackingzeng@vip.163.com）。

收稿日期：2019-08-06