| 第 49 卷第 6 期 |  | Vol. 49 No. 6 | | 2019 年 12 月 | Dec 2019 |
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所属栏目：功能材料
均匀设计法优化栀子苷类活性成分提取工艺及其应用 |
| 姜 红，向思孟，夏宇博，甘期望，刘琳琪*，赵晨曦
（长沙学院 生物与环境工程学院，湖南 长沙 410022） |
| 摘 要：以栀子苷的溶出率为指标，采用均匀设计U10×（108）考察了回流和超声法中各提取条件对栀子苷类活性成分溶出的影响，进行回归分析建立数学模型，得到优化条件。结果表明超声法明显优于回流法，确定了以70%乙醇按照20 mL·g-1液固比超声提取50 min的提取条件。将该提取工艺用于10批栀子和10批焦栀子药材的提取，结果发现，栀子药材中栀子苷含量4.26%~6.29%。各批次焦栀子中栀子苷均低于栀子，含量在3.45%~5.23%。由此可见，采用均匀设计法可快速、准确地确定栀子苷类活性成分的最佳提取工艺、条件，能更准确地评价和控制栀子药材质量。 |
| 关键词：栀子；栀子苷；均匀设计；超声波提取；高效液相色谱 |
| 中图分类号：R914.5 文献标识码：A 文章编号：1009-9212（2019）06-0049-05 |
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| Optimization and Application of Extraction Process of Iridoids Components from Gardeniae Fructus by Uniform Design |
| JIANG Hong, XIANG Si-meng, XIA Yu-bo, GAN Qi-wang, LIU Lin-qi*, ZHAO Chen-xi
(School of Biological and Environmental Engineering, Changsha University, Changsha 410022, China) |
| Abstract：In this paper, uniform design U10*(108) was used to investigate the factors affecting the release of iridoids components from Gardeniae Fructus in both reflux and ultrasonic extraction methods using the amount of gardenoside as evaluation criteria. The extraction conditions including time, solvent (ethanol) concentration and liquid-to-solid ratio were considered. Regression analysis were performed to establish mathematical models. The results showed that ultrasonic extraction method was obviously superior to the reflux extraction method. The optimal conditions for ultrasonic extraction process were obtained as following: 70% ethanol was used as solvent; the liquid-solid ratio was of 20 mL·g-1; and the extracting time was of 50 min. The extraction conditions were tested in extraction of 10 batches of Gardenia and 10 batches of coke Gardenia samples. The results showed that the iridoid glycosides constituents were different in different batches of gardenia medicinal materials. The contents of gardenoside varied from 4.26% to 6.29%. The contents of gardenoside in different batches of coke gardenia were lower than those in gardenia with a range of 3.45% to 5.23%. Thus, uniform design was successfully used to appraise the appropriate method and conditions for quick and accurate extraction of iridoid components. This presented a basis for quality evaluation and precise control of gardenia. |
| Key words：Gardeniae Fructus; Gardenoside; uniform design; ultrasonic extraction; HPLC |
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基金项目：湖南省自然科学基金项目（2017JJ3342），长沙市科技计划项目（kc1809036），2018年度长沙学院“大学生创新项目计划”，2019年度长沙学院创新创业训练计划项目。
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作者简介：姜 红（1999-），女，湖南宁乡人，研究方向：植物活性成分的研究（E-mail：2644356131@qq.com）。
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联 系 人：刘琳琪，实验师，研究方向：植物活性成分的研究（E-mail：linqi.liu@163.com）。
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收稿日期：2019-11-27
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