第 50 卷第 2 期 Vol. 50 No. 2 2020 年 4 月 Apr 2020

所属栏目：分析与测试 鸡尾酒中山梨酸钾的荧光检测方法研究

迟恩忠，赵雅婷，王 丽*，张 强，张 玲 （广东石油化工学院 生物与食品工程学院，广东 茂名 525000）

摘　要：为建立山梨酸钾的荧光检测新方法，研究了pH值、离子种类和浓度对山梨酸钾荧光特性的影响，优化了检测体系，建立了标准曲线法检测鸡尾酒中山梨酸钾的荧光检测方法，并对该方法进行了评价。结果表明，合适的检测pH值为11.5，体系中含0.1 g·L-1氯化钙，激发波长为358 nm，发射波长为458 nm。山梨酸钾浓度在0.01～1.00 g·L-1浓度范围内与荧光强度呈良好的线性关系，线性方程为y=1 863x+41.9，相关系数0.997 6，检出限（CL）0.006 7 g·L-1，相对标准偏差（RSD）0.47%。用于鸡尾酒中山梨酸钾的测定，加标回收率96.40%～98.80%。

关键词：鸡尾酒；山梨酸钾；荧光分光光度法；检测

中图分类号：TQ225.24　　文献标识码：A　　文章编号：1009-9212（2020）02-0063-04

Study on Fluorescence Detection Method of Potassium Sorbate in Cocktails

CHI En-zhong, ZHAO Ya-ting, WANG Li*, ZHANG Qiang, ZHANG Ling (College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, 525000 Maoming, China)

Abstract：In order to establish a fluorescent detection method for potassium sorbate, the fluorescence characteristic of potassium sorbate was studied in different pH and in the presence of different ions. The experimental conditions were optimized and the standard curve method was evaluated. The results showed that the optimal detection conditions were a pH value of 11.5, a CaCl2 concentration of 0.1 g·L-1, an excitation wavelength of 358 nm and an emission wavelength of 458 nm. Under the optimal conditions, the fluorescence intensity showed a linear relationship with the potassium sorbate concentration in the range between 0.01 and 1.00 g·L-1, and the linear equation was y=1 863x+41.9 with a correlation coefficient of 0.997 6. The limit of detection was 0.006 7 g·L-1 with a relative standard deviation of 0.47%. The recovery rates ranged from 96.40% to 98.80%, when the method was applied in the measure of potassium sorbate in cocktails.

Key words：cocktails; potassium sorbate; fluorescence spectrophotometry; determination

基金项目： 茂名市科技计划项目（2018049），广东省科技创新战略专项资金（KJ024），广东石油化工学院自然科学研究项目（2018rc40），广东普通高校食品科学创新团队项目（2016KCXTD020）资助。

作者简介：迟恩忠（1970-），男，山东蓬莱人，助理实验师，硕士，研究方向：食品加工与安全（E-mail：junfengchi@gdupt.edu.cn）。

联 系 人：王 丽，副教授，博士，研究方向：食品质量与安全（E-mail：wangli7742@gdupt.edu.cn）

收稿日期：2020-04-03