第 50 卷第 2 期 Vol. 50 No. 2 2020 年 4 月 Apr 2020

所属栏目：分析与测试 高效液相色谱-二极管阵列检测器法测定青霉素亚砜酯含量

王云华，王志强，朱 君，毕 弋，冉兆晋，焦佳媛，柴宝山* （沈阳化工研究院有限公司，辽宁 沈阳 110021）

摘　要：建立高效液相色谱-二极管阵列检测器法测定青霉素亚砜酯的含量。采用Agilent Eclipse Plus C18（3.0 mm×100 mm，3.5 μm）色谱柱；柱温30 ℃；以25 mmol·L-1的醋酸铵水溶液（A）-乙腈（B）为流动相进行梯度洗脱；流速为0.5 mL·min-1；检测波长267 nm。青霉素亚砜酯在0.01~0.80 mg·mL-1（R2=0.999 9）范围内线性较好，精密度RSD为0.15%。方法操作简单，结果准确，特异性强，稳定性好，适用于青霉素亚砜酯的含量测定。

关键词：青霉素亚砜酯；高效液相色谱；二极管阵列检测器；含量测定

中图分类号：O657.7+2　　文献标识码：A　　文章编号：1009-9212（2020）02-0067-03

Determination of Penicillin Sulfoxide Ester by High Performance Liquid Chromatography Coupling with Photodiode Array Detector

WANG Yun-hua, WANG Zhi-qiang, ZHU Jun, BI Yi, RAN Zhao-jin, JIAO Jia-yuan, CHAI Bao-shan* (Shenyang Research Institute of Chemical Industry Co.,Ltd., Shenyang 110021, China)

Abstract：A method was established for determination of penicillin sulfoxide ester by high performance liquid chromatography coupling with photodiode array detector. In this method Agilent Eclipse Plus C18(3.0×100 mm,3.5 μm) column was used and the temperature was set at 30 ℃. Gradient elution was performed with 25 mmol·L-1 ammonium acetate aqueous solution and acetonitrile as mobile phase. The flow rate was 0.5 mL·min-1 and the detection wavelength was 267 nm. The calibration curves of penicillin sulfoxide ester was linear in the range of 0.01~0.8 mg·mL-1(R2=0.999 9), and the RSD was 0.15%. This method is simple, accurate, highly specific and good stability, thus can be used for the determination penicillin sulfoxide ester.

Key words：penicillin sulfoxide ester; HPLC; DAD; content determination

作者简介：王云华（1990-），女，辽宁沈阳人，工程师，硕士，研究方向：药物分析。

联 系 人：柴宝山，高级工程师，博士，研究方向：药物化学（E-mail：chaibaoshan@sinochem.com）。

收稿日期：2020-04-08