第 51 卷第 4 期Vol. 51 No. 4
2021 年 8 月Aug 2021
所属栏目：分析与测试

三维荧光结合二阶校正法同时测定人体液中的两种抗丙型肝炎药物
童高焱，吴海龙*，常月月，王 童，谌露珠，俞汝勤 （湖南大学 化学化工学院 化学生物传感与计量学国家重点实验室，湖南 长沙 410082）
摘　要：提出一种三维荧光结合基于交替三线性分解（ATLD）算法的二阶校正方法快速对人体血清和尿液中的两种抗丙型肝炎药物达卡他韦（DAC）和雷迪帕韦（LED）进行定性定量的分析方法。二阶校正方法因具有“二阶优势”，可避免复杂的样本预处理，即使分析物之间和分析物与未知干扰之间存在严重的光谱重叠，该方法仍可得到满意的定性定量结果。两种药物的平均回收率为98.35%~109.95%，相关系数大于0.99。同时，相应的品质因子参数反映出该方法具有较高的灵敏度，较好的选择性和较低的检测限。这一方法的建立有望为临床药物的快速分析提供新思路。
关键词：三维荧光；二阶校正；交替三线性分解；二阶优势；丙型肝炎
中图分类号：R969　　文献标识码：A　　文章编号：1009-9212（2021）04-0054-07
Simultaneous Determination of Two Anti-HCV Drugs in Human Fluids Using Three-way Fluorescence Coupled with Second-order Calibration Method
TONG Gao-yan, WU Hai-long*, CHANG Yue-yue, WANG Tong, CHEN Lu-zhu, YU Ru-qin (State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China)
Abstract：This work proposed an analytical method that three-way fluorescence coupled with second-order calibration based on alternative trilinear decomposition (ATLD) algorithm to rapidly and simultaneously quantify two anti-hepatitis C drugs including daclatasvir (DAC) and ledipasvir (LED) in human serum and urine. Using the "second-order advantage" of the second-order calibration method can avoid complicated sample pretreatment. Even if the fluorescence spectra of interested analytes overlapped and unknown interference coexisted in the system, the method can still require satisfactory qualitative and quantitative results. The average recoveries were 98.35%~109.95%, and the correlation coefficients were more than 0.99. The figures of merit also showed that the method has higher sensitivity and better selectivity and lower limit of detection. The establishment of this method is expected to provide new ideas for the rapid determination of clinical drugs.
Key words：three-way fluorescence; second-order calibration; alternative trilinear decomposition; second-order advantage; hepatitis C
基金项目：国家自然科学基金资助项目（21775039，2217040266，21521063）。
作者简介：童高焱（1996-），女，云南昭通人，硕士研究生，主要从事化学计量学多维校正理论及应用（E-mail：gytong@hnu.edu.cn）。
联 系 人：吴海龙，教授，主要从事化学计量学多维校正理论及应用相关研究（E-mail：hlwu@hnu.edu.cn）。
收稿日期：2021-08-23