第 51 卷第 4 期 Vol. 51 No. 4 2021 年 8 月 Aug 2021

所属栏目：分析与测试 吡噻菌胺及其代谢物在番茄中残留分析方法研究

孙 炯，任 竞*，傅 强，付启明，冯 雪，何 鑫，杨 柳，周诗瑜 （湖南文谱检测技术研究有限公司，湖南 长沙 410137）

摘　要：采用QuEChERS前处理方法结合高效液相色谱-串联质谱（UPLC-MS／MS）多反应监测（MRM）模式测定吡噻菌胺及其代谢物1-甲基-3-（三氟甲基）-1H-吡唑-4-甲酰胺（PMA）在番茄中的残留量。样品经0.2%甲酸乙腈提取，N-丙基乙二胺（PSA）和无水硫酸镁净化，UPLC-MS／MS检测。方法在0.000 1~0.6 mg／L范围内有良好的线性关系，番茄中吡噻菌胺和1-甲基-3-（三氟甲基）-1H-吡唑-4-甲酰胺（PMA）最低检出量均为3×10-13 g，定量限为0.002 mg／kg。样本中添加量为0.002、0.01、0.1、1.0、2.0 mg／kg时（n=5），平均回收率92.6%~104.8%，相对标准偏差1.3%~2.7%。

关键词：吡噻菌胺；PMA；QuECh ERS；高效液相色谱-串联质谱；残留

中图分类号：O657.7　　文献标识码：A　　文章编号：1009-9212（2021）04-0061-04

Analytic Method for the Residue of Penthiopyrad and Its Metabolite PMA in Tomata

SUN Jiong, REN Jing*, FU Qiang, FU Qi-ming, FENG Xue, HE Xin, YANG Liu, ZHOU Shi-yu (Hunan Winp Analytical Technology Research Co.,Ltd., Changsha 410137, China)

Abstract：The residues of Penthiopyrad and its metabolite PMA in tomato were investigated by QuEChERS-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)(MRM). Samples were prepared through extraction using solvent of 0.2% formic acid in acetonitrile, and purified by both PSA and MgSO4, were analyzed by UPLC-MS/MS. The linear range of the method was from 0.000 1 to 0.6 mg/L with a determination coefficient (R2) of more than 0.99. The limit of detection (LOD) for both Penthiopyrad and PMA was of 3×10-13 g, and the limit of quantitation (LOQ) was of 0.002 mg/kg. At the four levels including 0.002, 0.01, 0.1, 1.0 and 2.0 mg·kg-1, the average recovery of the penthiopyrad and PMA was between 92.6% and 104.8%, the RSD were between 1.3% and 2.7%.

Key words：Penthiopyrad; PMA; QuEChERS; UPLC-MS/MS; detecting residues

作者简介：孙 炯（1984-）男，湖南宁乡人，硕士，研究方向：农药残留检测、农药质量检测（E-mail：sunjiong2010@163.com）。

联 系 人：任竞，助理研究员，硕士，研究方向：农药残留分析（E-mail: renjing-1980@sina.cn）。

收稿日期：2021-05-27