第 51 卷第 4 期 Vol. 51 No. 4 2021 年 8 月 Aug 2021

所属栏目：分析与测试 GC内标法测定固精麦斯哈片中丁香酚含量的研究

赵 敏，姜艳玲，王莉莉，严 力 （西安万隆制药股份有限公司，陕西 杨凌 712100）

摘　要：采用气相色谱内标法，以HP-INNOWAX毛细管柱（30 μm×530 μm×1.00 μm）为色谱柱，FID检测器，等温法（185 ℃），溶剂直接进样，建立了固精麦斯哈片中丁香酚含量的测定方法。结果表明：丁香酚在0.723 5~2.804 0 mg／mL范围内线性关系良好，R2=0.999 7（n=5），平均回收率 99.36%，RSD为0.69%（n=6），重复性实验 RSD为0.51%（n=6）。所建立的方法空白溶剂无干扰、稳定性好、准确度高、适用性强、结果可靠且易于操作，适用于测定固精麦斯哈片中丁香酚含量的测定。

关键词：内标法；固精麦斯哈片；丁香酚含量；气相色谱法

中图分类号：O657.7+1　　文献标识码：A　　文章编号：1009-9212（2021）04-0073-04

Determination of Eugenol in Gujing Maisiha Tablets by GC Internal Standard Method

ZHAO Min, JIANG Yan-ling, WANG Li-li, YAN Li (Xi'an WanLong Pharmaceutical Co.,Ltd., Yangling 712100, China)

Abstract：A method for determination of eugenol content in Gujing Maisiha tablet was established using using Gas chromatography equipped with HP-INNOWAX capillary column (30 μm×530 μm×1.00 μm) and FID detector. Isothermal method (185 ℃), direct solvent injection, internal standard method were also selected in this analytical process. The results showed as followings: Eugenol had a good linear relationship in the range of 0.723 5~2.804 0 mg/mL, R2=0.999 7(n=5); the average recovery rate was 99.36%; RSD was 0.69%(n=6); RSD was 0.51% (n=6). This established method has advantages including no interference, good stability, high accuracy, strong durability, reliable results and easy operation. It is suitable for the determination of eugenol content in Gujing Maisiha tablets.

Key words：internal standard method; gujingmaisiha tablet; eugenol content; gas chromatography

作者简介：赵 敏（1989—），女，陕西杨凌人，执业药师，主要从事药物分析和药品质量管理工作。

收稿日期： 2021-06-0