第 51 卷第 5 期 Vol. 51 No. 5 2021 年 10 月 Oct 2021

所属栏目：分析与测试 环丙氟虫胺原药高效液相色谱分析

吴 若1，肖瑛子1，胡金蕾1，唐 亮1，王 丹1，庞怀林2 （1. 长沙嘉桥生物科技有限公司，湖南 长沙410209；2. 南通泰禾化工股份有限公司，江苏 南通 226407）

摘　要：研究了高效液相色谱测定环丙氟虫胺原药含量的HPLC方法。采用C18不锈钢色谱柱（150 mm×4.6 mm i.d.× 5 μm）和紫外检测器，以甲醇-0.1%磷酸水溶液（V∶V=75∶25）为流动相，流速为1.5 mL／min，在240 nm波长下，柱温为40 ℃±2 ℃，进样体积为10 μL。在上述色谱操作条件下，对环丙氟虫胺原药进行了定量分析。考察了分析方法特异性、线性回归方程、精密度和准确度。该方法测得环丙氟虫胺的保留时间约8.6 min，线性相关系数为0.999 8，标准偏差0.10，变异系数0.10%，平均回收率99.20%。

关键词：环丙氟虫胺；高效液相色谱；分析

中图分类号：O657.7+2　　文献标识码：A　　文章编号：1009-9212（2021）05-0093-03

Analysis of Cyproflanilide TC by HPLC

WU Ruo1, XIAO Ying-zi1, HU Jin-lei1, TANG Liang1, WANG Dan1, PANG Huai-lin2 (1. Changsha Jia-qiao Biotech Co.,Ltd., Changsha 410209, China；2. CAC Nantong Chemicals Co., Ltd., Nantong 226407, China)

Abstract：The content of Cyproflanilide was determined using HPLC. In this method, the sample was analyzed using a stainless steel column (150 mm×4.6 mm i.d.×5 μm) packed with C18 filler equipped with a UV detector of 240 nm; a mixture of methanol and 0.1% phosphoric acid aqueous solution was used as mobile phase at a flow rate of 1.5 mL/min. The factors including specificity, linear regression equation, precision and accuracy of the method were investigated. Ruselts showed that the retention time of Cyproflanilide was 8.6 min； the linear correlation was of 0.999 8；a standard deviation was of 0.10; a variation coefficient was of 0.10%, and an average recovery of 99.20% were obtained. The results showed that this method was sensitive, simple and rapid, and suitable for the quantitative analysis of cyproflanilide.

Key words：cyproflanilide; HPLC; analysis

作者简介：吴 若（1987—），男，安徽安庆人，研究方向：农药分析方法的研究及标准编写（E-mail：ruo_wu@cacch.com）

收稿日期：2021-08-03