第 52 卷第 3 期 Vol. 52 No. 3 2022 年 6 月 Jun 2022

所属栏目：分析与测试 高效液相色谱法定量分析工业废水中正丁醇

赵军星 （青岛市排水运营服务中心，山东 青岛 266000）

摘　要：建立了定量测定工业废水中正丁醇高效液相色谱法。以月旭Xtimate C-18色谱柱（4.6 mm×250 mm，5 μm）分离，甲醇-水（V∶V=30∶70）为流动相，流速1.0 mL/min，柱温30 ℃，进样量10 μL，折光率视差检测器检测。结果表明，正丁醇质量浓度在79.93~39 965 mg/L范围内与峰面积线性关系良好（R2=0.999 1，n=7），检测限和定量限分别为24.9 mg/L和49.9 mg/L，精密度实验相对标准偏差为3.84%，回收率在90%以上。该方法样品前处理简单，分析过程快捷，结果准确，重复性好，可用于工业废水中正丁醇含量测定。

关键词：高效液相色谱法；正丁醇；含量测定

中图分类号：X703　　文献标识码：A　　文章编号：1009-9212（2022）03-0082-03

Determination of n-Butanol in Industrial Wastewater by HPLC

ZHAO Jun-xing (Qingdao Drainage Operations Service Center, Qingdao 266000, China)

Abstract：A high performance liquid chromatography (HPLC) method for the determination of n-butanol in industrial wastewater was established. In this method, the chromatography column was Welch Xtimate C-18 column (4.6 mm×250 mm, 5 μm); the mobile phase was methanol-water (V∶V=30∶70); the flow rate was 1.0 mL/min; the column temperature was 30 ℃; the injection volume was 10 μL; and the detector was refractive index parallax detector. The results showed that the linear range of n-butanol was 79.93~39 965 mg/L (R2=0.999 1, n=7)．The limit of detection and limit of quantitation of n-butanol were 24.9 mg/L and 49.9 mg/L, respectively. The RSD was 3.84% and the recovery rate was above 90%. This method has the advantages of simple pretreatment, fast analysis process, accurate analysis result and fgood repetition, and can be used for the determination of n-butanol content in industrial wastewater.

Key words：HPLC; n-butanol; content determination

作者简介：赵军星（1964—），女，山东莱阳人，高级工程师，研究方向：环境监测及质量控制（E-mail：584459514@qq.com）。

收稿日期：2021-12-17