第 52 卷第 4 期Vol. 52 No. 4
2022 年 8 月Aug 2022
所属栏目：分析与测试

气相色谱法测定和厚朴酚原料药中残留溶剂的研究
陈莉莉1，杨云霞2，门 靖3 （1. 陕西大河药业有限责任公司，陕西 西安 710200；2. 陕西省药品和疫苗检查中心咸阳分中心，陕西 咸阳 712000；3. 西安万隆制药股份有限公司，陕西 西安 710119）
摘　要：采用气相色谱法，以二甲基亚砜为溶剂，顶空进样，以DB-624毛细管柱程序升温，FID检测器，建立了和厚朴酚中乙醇、二氯甲烷、正己烷等残留溶剂的测定方法。结果表明：乙醇在2.500 4~750.120 μg/mL范围线性方程为y=0.800 9x+4.133 2，相关系数0.999 1，线性良好；二氯甲烷在2.422~90.825 μg/mL范围线性方程y=0.894 2x-0.639，相关系数0.999 5，线性良好；正己烷在0.096 469~43.845 μg/mL范围线性方程为y=42.072x-29.422，相关系数0.995 5，线性良好。所建立的方法灵敏度高，准确性好，操作简便，适用于和厚朴酚残留溶剂的测定。
关键词：和厚朴酚；残留溶剂；顶空进样；气相色谱法
中图分类号：R284.1　　文献标识码：A　　文章编号：1009-9212（2022）04-0084-05
Determination of Residual Solvents in Honokiol by Gas Chromatography
CHEN Li-li1, YANG Yun-xia2, MEN Jing3 (1. Shaanxi Dahe Pharmaceutical Co., Ltd., Xi’an 710200, China; 2. Xianyang Branch of Shaanxi Province Drug and Vaccine Inspection Center, Xiangyang 712000, China; 3. Xianyang Wanlong Pharmaceutical Co., Ltd., Xi’an 710119, China)
Abstract：In a gas chromatography method, using the dimethyl sulfoxide as solvent, adopting the headspace sampling method, raising the temperature through DB-624 capillary column program, FID as the detector, the residual solvent such as ethanol, dichlormethane and n-hexane in honokiol was detected. The results showed that: the linear equation was expressed as y=0.800 9x+4.133 2 when ethanol is in the range of 2.500 4~750.120 μg/mL, the correlation coefficient reached 0.999 1, showing good linearity. The linear equation was expressed as y=0.894 2x-0.639 when dichloromethane falled in the range of 2.422~90.825 ug/mL, the correlation coefficient reached 0.999 5, with good linearity. And it was expressed as y=42.072x-29.422 when n-hexane falled in the range of 0.096 469~43.845 μg/mL, also showing the correlation coefficient of 0.995 5 and good linearity. This method was suitable for the determination of honokiol and honokiol residual solvents with the advantages including high sensitivity, good accuracy and simple operation.
Key words：honokiol; residual solvent; headspace sampling; gas chromatography method
作者简介：陈莉莉（1980—），女，陕西蒲城人，主管药师，执业药师，研究方向：医药研发、植物提取及中药化学研究（E-mail：695766755@qq.com）。
收稿日期：2022-04-19