第 52 卷第 5 期 Vol. 52 No. 5 2022 年 10 月 Oct 2022

所属栏目：农药及中间体 湖南不同地区草地贪夜蛾种群对四种类型杀虫剂的抗药性监测

黄至畅1，2，3，龙楚云1，2，3，吴明峰1，2，3，钟馥骏1，2，3，吴晓峰4，李建明1，2，3，欧晓明1，2，3* （1. 湖南化工研究院有限公司 国家农药创制工程技术研究中心，湖南 长沙 410014；2. 农用化学品湖南省重点实验室，湖南 长沙 410014；3. 湖南化研院检测技术有限公司，湖南 长沙 410014；4. 永州市农业科学研究所，湖南 永州 431000）

摘　要：为明确湖南草地贪夜蛾对甲氨基阿维菌素苯甲酸盐、氯虫苯甲酰胺、高效氯氟氰菊酯和茚虫威等的抗药性程度，建立了田间玉米草地贪夜蛾种群的敏感基线。用浸叶法测定了四种类型杀虫剂对湖南省10个地级市的草地贪夜蛾种群3龄幼虫的LC50，并以此为毒力基线。将10个种群所测数据进行合并计算毒力回归方程，得出混合种群的敏感基线。结果表明草地贪夜蛾不同田间种群对四种类型的敏感性均存在一定差异，其中对甲氨基阿维菌素苯甲酸盐抗性最低和抗性最高的种群分别为张家界（慈利县）种群和常德（鼎城区）种群，其抗性倍数分别为4.95和2.21，最高值与最低值之间的抗性差异达到2.24倍，10地种群对四种类型的抗性倍数在1~5之间。湖南不同地区草地贪夜蛾种群对四种类型杀虫剂虽未达到低抗性水平，但敏感性已降低。

关键词：草地贪夜蛾；敏感基线；抗药性

中图分类号：S433.4　　文献标识码：A　　文章编号：1009-9212（2022）05-0030-05

Monitoring the Resistance of Spodoptera frugiperda to Four Types of Insecticides in Different Areas of Hunan Province

HUANG Zhi-chang1,2,3, LONG Chu-yun1,2,3, WU Ming-feng1,2,3, ZHONG Fu-jun1,2,3, WU Xiao-feng4, LI Jian-ming1,2,3, OU Xiao-ming1,2,3 (1. National Engineering Research Center for Agrochemicals, Hunan Research Institute of Chemical Industry, Co., Ltd., Changsha 410014, China; 2. Hunan Provincial Key Laboratory for Agrochemicals, Changsha 410014, China; 3. Hunan Chemical Research Institute Testing Technology Co., Ltd., Changsha 410014, China; 4. Yongzhou Agricultural Science Research Institute, Yongzhou 431000, China)

Abstract：In order to determine the degree of resistance of Spodoptera frugiperda in Hunan Province to emamectin benzoate, chlorantraniliprole, lambda-cyhalothrin and indoxacarb, a sensitive baseline of Spodoptera frugiperda population in corn grassland was established. The LC50 of four types of insecticides against the 3rd instar larvae of Spodoptera frugiperda gracilis collected from 10 prefecture level cities in Hunan province was determined by leaf dipping method, and this was taken as the toxicity baseline. The sensitive baseline of the mixed population was obtained by combining the measured data of 10 populations to calculate the virulence regression equation. The results showed that there were some differences in the sensitivity of different field populations to the four types. The populations with the lowest and highest resistance to methylaminoabamectin benzoate were Zhangjiajie (Cili County) population and Changde (Dingcheng District) population, their resistance multiples were 4.95 and 2.21, respectively. The difference between the highest and lowest resistance values was 2.24 times. The resistance times of 10 populations to the four types ranged from 1 to 5. Although the population of Spodoptera frugiperda in different areas of Hunan has not reached the low resistance level to the four types of insecticides, its sensitivity has decreased.

Key words：Spodoptera frugiperda; baseline sensitive; drug resistance

基金项目：湖南省自然科学基金，青年基金资助项目（2020JJ5146）。

作者简介：黄至畅（1989—），男，湖北孝感人，研究方向：植物保护（E-mail：591611226@qq.com）。

联 系 人：欧晓明，研究员，博士，研究方向：农药残留化学、环境毒理学（E-mail：xmouhn@163.com）。

收稿日期：2022-10-09