第 52 卷第 5 期Vol. 52 No. 5
2022 年 10 月Oct 2022
所属栏目：分析与测试

二氯异丙虫酰胺的高效液相色谱分析
刘小锋1，2，3，陈 颖1，2，3，陈一萍1，2，3 （1. 湖南化工研究院有限公司 国家农药创制工程技术研究中心，湖南 长沙 410014；2. 农用化学品湖南省重点实验室，湖南 长沙 410014；3. 湖南化研院检测技术有限公司，湖南 长沙 410014）
摘　要：建立了二氯异丙虫酰胺的高效液相色谱分析方法。样品用乙腈溶解，采用Agilent TC-C18柱为色谱柱，V（乙腈）∶V（水）=50∶50为流动相，PDA检测器在245 nm对二氯异丙虫酰胺进行高效液相色谱分离和检测。方法在13.6~680 µg/mL的浓度范围内线性关系良好，线性回归方程为y=22 659x-15 086，相关系数R2=0.999 9，变异系数为0.37，平均回收率为99.9%。该方法简单可靠，精密度及准确度符合定量分析要求，可用于二氯异丙虫酰胺的定量分析。
关键词：高效液相色谱；二氯异丙虫酰胺；分析
中图分类号：O657.7+2　　文献标识码：A　　文章编号：1009-9212（2022）05-0088-03
Determination of 3-Bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(isopropylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide by HPLC
LIU Xiao-feng1,2,3, CHEN Ying1,2,3, CHEN Yi-ping1,2,3 (1. National Engineering Reserch Center for Agrochemicals, Hunan Research Institute of Chemical Industry Co., Ltd., Changsha 410014, China; 2. Hunan Province Key Laboratory for Agrochemicals, Changsha 410014, China; 3. Hunan Chemical Research Institute Testing Technology Co., Ltd., Changsha 410014, China)
Abstract：To establish a high performance liquid chromatography method for 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(isopropylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide, the samples dissolved in acetonitrile were separated using reverse phase column Agilent TC-C18 as chromatographic column with a mobile phase of acetonitrile to water volume ratio of 50∶50; PDA detector was selected to separate and read the signal of compound at 245 nm. This method could obtain compound’s signal nicely with a linear model between 13.6~680 µg/mL of concentration. The regressed linear was y=22 659x-15 086 and the regression coefficiency R² was of 0.999 9; the coefficient of variant was of 0.37; the average recovery was of 99.9%. This method was simple, stable and accurate, and meet the requirements for quantitative analysis of 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(isopropylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide.
Key words：HPLC; 3-bromo-1-(3-chloropyridin-2-yl)-N-(2,4-dichloro-6-(isopropylcarbamoyl)phenyl)-1H-pyrazole-5-carboxamide; analysis
作者简介：刘小锋（1993—），男，湖南邵东人，研究方向：农药分析（E-mail：411388486@qq.com）。
收稿日期：2022-09-01