| 第 53 卷第 1 期 |  | Vol. 53 No. 1 | | 2023 年 2 月 | Feb 2023 |
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所属栏目：医药及中间体
不同炮制温度对盐杜仲主要有效成分含量的影响 |
| 管松滨1，胥 骞2，卢白荟2，龚 妹1，彭东明2*，卢茂芳2
（1.湖南振兴中药有限公司，湖南 长沙 410299；2. 湖南中医药大学 药学院，湖南 长沙 410208） |
| 摘 要：比较盐杜仲不同温度下炮制前后主要化学成分松脂醇二葡萄糖苷的变化，推测其适宜炮制温度并优化炮制工艺参数。依据《中华人民共和国药典》（2020年版）中的“断丝”指标，考察不同炮制温度对杜仲“断丝”程度的影响，优化杜仲炮制工艺参数。采用高效液相色谱（HPLC）分析杜仲生品和盐炙品主要化学成分的差异，研究炮制工艺对杜仲质量的影响。结合实验数据确定杜仲炮制温度为110 ℃，时间控制在8 min左右。高温炮制后杜仲化学成分含量明显降低，其主要有效成分松脂醇二葡萄糖苷（PDG）也大幅减少。采用HPLC方法，分析炮制前后杜仲主要化学成分的变化，为杜仲盐炙工艺和盐杜仲的质量控制提供了更可行的参数依据。 |
| 关键词：盐杜仲；炮制温度；HPLC分析；松脂醇二葡萄糖苷 |
| 中图分类号：R917 文献标识码：A 文章编号：1009-9212（2023）01-0034-05 |
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| Effects of Processing Temperatures on the Content of Main Active Components in Salt Eucommia |
| GUAN Song-bin1, XU Qian2, LU Bai-hui2,GONG Mei2, PENG Dong-ming2*, LU Mao-fang2
(1. Hunan Zhenxing Traditional Chinese Medicine Co., Ltd., Changsha 410299, China;2. College of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China） |
| Abstract：Based on the changes of the main chemical contents in pinoresinol diglucoside (PDG) before and after processing at different temperatures of Salt Eucommia the optimum processing parameters were determined. According to the "broken silk" index in "Pharmacopoeia of the People's Republic of China" (2020 edition), the effect of different processing temperatures on the degree of "broken silk" of Eucommia was investigated. Using High performance liquid chromatography (HPLC) to analyze the difference of main chemical contents between raw Eucommia ulmoides and salted products the effect of processing technology on the quality of Eucommia ulmoides was studied. Based on the experimental data it was determined that the optimum temperature for Eucommia ulmoides processing was 110 ℃, and the time was controlled at about 8 min. After high temperature processing, the contents of chemical components of Eucommia ulmoides was significantly reduced, and the main active ingredient pinoresinol diglucoside was also greatly reduced. |
| Key words：Salt Eucommia; processing temperature; HPLC analysis; pinoresinol diglucoside |
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基金项目：湖南省卫生健康委科研重点课题（No.202113052006），湖南省自然科学科药联合基金项目（No.2021JJ80011），湖南省药学一流学科开放基金（科函〔2020〕4号－2020YX01、2020YX07、2021YX12）。
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作者简介：管松滨（1969—），男，湖南武冈人，主管中药师，研究方向：中药炮制与加工（E-mail：157011995@qq.com）。
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联 系 人：彭东明，教授，博士，研究方向：新药研究与开发（E-mail：pdmcsu@126.com）。
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收稿日期：2022-06-22
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