| 第 53 卷第 2 期 |  | Vol. 53 No. 2 | | 2023 年 4 月 | Apr 2023 |
|
所属栏目：分析与测试
LC-MS液质联用法测定白酒中甜蜜素的含量 |
| 周远明，胡景田，陈贵兰，王倩文*
（青岛农业大学 分析测试中心，山东 青岛 266109） |
| 摘 要：白酒样品用超纯水直接稀释，采用10 mmoL/L乙酸铵水溶液和甲醇作为流动相，梯度洗脱，液质联用法测定白酒中甜蜜素含量。结果表明，甜蜜素质量浓度在0.7~14 μg/mL范围内线性关系良好（R2=0.995，n=6），检测限和定量限分别为0.6 μg/kg及3 μg/kg，精密度实验相对标准偏差为9.7%，回收率在70%以上。该方法样品前处理简单，可以减少污染风险，分析过程快捷准确，重复性好，可用于白酒中甜蜜素的含量测定。 |
| 关键词：白酒；甜蜜素；液质联用法 |
| 中图分类号：O656.3 文献标识码：A 文章编号：1009-9212（2023）02-0079-05 |
|
| Content Determination of Sodium Cyclamate in Liquor by High Performance Lliquid Chromatography-tandem Mass Spectrometry |
| ZHOU Yuan-ming, HU Jing-tian, CHEN Gui-lan, WANG Qian-wen*
(Analytical and Testing Center, Qingdao Agricultural University, Qingdao 266109, China) |
| Abstract：Liquor sample was directly diluted with ultrapure water, liquid chromatography-tandem mass spectrometry method was used to determine sodium cyclamate content in liquor with methanol and 10 mmoL/L ammonium acetate water solution as mobile phase for gradient elution. The results show that the linear range of sodium cyclamate was 0.7~14 μg/mL (R2=0.995, n=6)．The limit of detection and limit of quantitation of sodium cyclamate were 0.6 μg/kg and 3 μg/kg, respectively. The RSD value was 9.7%, the recovery rate was above 70%. This method has the advantages of simple sample pretreatment with the reduction of the sample contamination likelihood, fast analysis process and fine repetition. So this method could be used for determination of the sodium cyclamate content in liquor. |
| Key words：liquor; sodium cyclamate; liquid chromatography-tandem mass spectrometry |
|
基金项目：青岛农业大学高层次人才科研基金（663/1119027）。
|
作者简介：周远明（1974—），男，湖北随州人，高级实验师，博士，研究方向：天然药物化学（E-mail：zym7410@163.com）。
|
联 系 人：王倩文，高级实验师，博士，研究方向：仪器分析方法的开发与研究（E-mail：wangqianwen_ac@126.com）。
|
收稿日期：2023-01-13
|
|