第 54 卷第 1 期 Vol. 54 No. 1 2024 年 2 月 Feb 2024

所属栏目：医药及中间体 酶法头孢拉定母液冷冻浓缩提取工艺研究

郭永刚1，刘 华2，张立斌3，4，5*，任 峰3，4，5，王利杰3，4，5，张建丽3，4，5 （1. 河北省药品职业化检查员总队（南片区），河北 石家庄 050000；2. 河北省药品职业化检查员总队（北片区），河北 秦皇岛 066100；3. 华北制药河北华民药业有限责任公司，河北 石家庄 052165；4. 抗生素酶催化与结晶技术国家地方联合工程实验室，河北 石家庄 052165；5. 河北省头孢类药物制备技术创新中心，河北 石家庄 052165）

摘　要：以头孢拉定离心母液为研究对象，研究了冷冻浓缩提取头孢拉定工艺，考察了冷冻装置、冷冻温度、pH、冷冻时间及搅拌方式等因素对冷冻浓缩效果的影响。结果表明：采用内刮式冷冻浓缩装置进行冷冻浓缩，冷冻温度-8 ℃，冷冻时间4 h，搅拌转速150 r/min时，冷冻浓缩效果较好，头孢拉定提取率88.7%。

关键词：头孢拉定；母液；冷冻浓缩

中图分类号：TQ460.6　　文献标识码：A　　文章编号：1009-9212（2024）01-0037-04

Study on the Freeze Concentration Process of Enzymatic Cefradine Mother Liquor

GUO Yong-gang1, LIU Hua2, ZAHNG Li-bin3,4,5*, REN Feng3,4,5, WANG Li-jie3,4,5, ZHANG Jian-li3,4,5 (1. Hebei Pharmaceutical Professional Inspector Corps (South Region), Shijiazhuang 050000, China; 2. Hebei Pharmaceutical Professional Inspector Corps (North Region), Qinhuangdao 066100, China; 3. NCPC Hebei Huamin Pharmaceutical Co., Ltd., Shijiazhuang 052165, China; 4. National and Local Joint Engineering Laboratory of Antibiotic Enzyme Catalysis and Crystallization Technology, Shijiazhuang 052165, China; 5. Hebei Province Cephalosporide Medicine Preparation Technology Innovation Center, Shijiazhuang 052165, China)

Abstract：To conduct experimental research on freeze concentration, cefradine centrifuge mother liquor was used as the research object. The effects of factors incuding freezing device, freezing temperature, pH value, freezing time, and stirring mode on the freezing process were studied. The results showed that using an internal scraping type freeze concentration device for freeze concentration, at a freezing temperature of -8 ℃, a freezing time of 4 hours, and a stirring speed of 150 r/min, the better freeze concentration effect was achieved, and the extraction yield of cefradine was 88.7%.

Key words：cefradine; mother solution; freeze concentration

作者简介：郭永刚（1982—），男，河北石家庄人，硕士，研究方向：药物化学合成研究（E-mail：13780300521@163.com）。

联 系 人：张立斌，高级工程师，研究方向：抗生素类药物合成及产业化研究（E-mail：119783274@qq.com）。

收稿日期：2023-12-01