| 第 33 卷第 5 期 |  | Vol. 33 No. 5 | | 2003 年 10 月 | Oct 2003 |
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所属栏目：其它
应用固定化青霉素酰化酶IPA-750制备7-ADCA的工艺研究 |
| 柳杏辉; 莫章桦; 曾红宇(福来格生物技术有限公司; 湖南长沙) |
| 摘 要：详细讨论了头孢G酸 (扩环酸 )在固定化青霉素酰化酶 (IPA 75 0 )催化裂解下转化为 7 ADCA的工艺。在 0.05mol/L的硼酸缓冲液中 ,通过改变酶促反应过程中底物浓度、pH值、温度 ,筛选出了优惠反应条件 :底物浓度 6 % ,pH8.0 ,温度 28～ 30℃。在优惠反应条件下进行批次反应 ,400～450批 ,转化率≥ 97% ,收率≥ 95 %。 |
| 关键词：固定化青霉素酰化酶; 头孢G酸; 7-ADCA; 酶促反应条件 |
| 中图分类号：TQ465.1 文献标识码：A 文章编号：1009-9212(2003)05-0037-02 |
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| A Study on Preparation of 7-ADCA Using Immobilied Penicillin Acylase |
| LIU Xin-hui; etc. （FLAG BIO-TECH.CO.; Ltd; Changsha Hunan 410100; China） |
| Abstract：It was particularly discussed that 7-(phenylacetamido)deace-toxycephalosporin acid was converted to 7-ADCA with catalytic decompostion of immobilied penicillin acylase. The optimum reaction conditions were obtained by adjusting substrate concentration, temperature, and pH in the buffer solution of 0.05?mol/L H_3BO_3. The reaction can be repeated for 400～450 cycles under the optimum reaction conditions of substrate concentration 6%, pH 8.0 , and temperature 28～30?℃。 The conversion rate is over 97% and the yi... |
| Key words：immobilized penicillin acylase; 7-（phenylacetamido ） deacetoxycephalosporin; 7-ADCA; enzymiatic condition |
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收稿日期：2003-06-23
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