第 35 卷第 2 期 Vol. 35 No. 2 2005 年 4 月 Apr 2005

所属栏目：分析与测试 α-氰基-3-苯氧基苄醇的HPLC分离与测定

罗积杏; 唐世平; 杨立荣(上海市农药研究所; 浙江大学生物工程研究所; 200032; 浙江杭州; 310027)

摘　要：利用手性色谱柱SumichiralOA4400,及流动相正己烷:二氯乙烷:乙醇=87∶10∶3(v/v),在流速1.0ml·min1,检测波长254nm,柱温15℃的分析条件下,成功实现了对映体(氰基3苯氧基苄醇的手性分离和定量分析,(S)异构体和(R)异构体的线性相关系数分别为0.9981和0.9975,变异系数分别为0.63%和0.64%,回收率分别为98.7%～101.3%和99.1%～99.9%。

关键词：α-氰基-3-苯氧基苄醇; 高效液相色谱; 分离; 测定

中图分类号：T255　　文献标识码：A　　文章编号：1009-9212(2005)02-0071-03

The separation and determination of α-cyano-3-phenoxybenzyl alcohol by HPLC

LUO Ji-xing ~（1）; TANG Shi-ping~（2）; YANG Li-rong~（2）（1.Shanghai Pesticide Research Institute; Shanghai 200032; China; 2.Institute of Bioengineering; Zhejiang University; Hangzhou 310027; China）

Abstract：Using normal HPLC module, α-cyano-3-phenoxy benzyl alcohol was excellent separated and its isomers were quantitative determined on a Sumichiral OA-4400 column, using n-hexane, 1,2-Dichloroethane and ethanol with volume rate 87∶10∶3 as mobile phase, under optimized conditions : detection wavelength 254 nm, mobile phase flow rate 1.0ml·min~(-1), and column temperature 15℃. The variation coefficients of the(S)- and(R)-enantiomer were 0.63% and 0 .64%, and the linear correlations were 0.9981 and 0.9975, the rec...

Key words：α-cyano-3-phenoxybenzyl alcohol; HPLC; separation; determination

收稿日期：2004-03-26

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