第 35 卷第 6 期 Vol. 35 No. 6 2005 年 12 月 Dec 2005

所属栏目：医药及中间体 HPLC法测定-α乙酰氨基-α-乙酯基-β-(3-吲哚)-丙酸乙酯

丁玲玲; 王守凯; 赵海(中钢集团鞍山热能研究院; 辽宁鞍山)

摘　要：采用Hypersill BDS C18色谱柱,以甲醇-水(60:40)为流动相,流速1.0 mL.min-1,233nm为检测波长,建立测定α-乙酰氨基-α-乙酯基-β-(3-吲哚)-丙酸乙酯的高效液相色谱法。进样量在4～20μg范围内线性关系良好(r=0.9999)。RSD不大于0.68%。方法操作简单,分析速度快、准确,适用于α-乙酰氨基-α-乙酯基-β-(3-吲哚)-丙酸乙酯的质量控制分析。

关键词：α-乙酰氨基-α-乙酯基-β-（3-吲哚）-丙酸乙酯; HPLC; 分析

中图分类号：TQ225.24+1　　文献标识码：A　　文章编号：1009-9212(2005)06-0037-02

Determination of α-Acotamino-α-Carbethoxy-β-(3-indole)-propionate by HPLC

DING Ling-ling; WANG Shou-kai （China Steel Group Anshan Research Institute Of Thermo-Energy; Anshan 114004; China）

Abstract：A HPLC method was established for the determination of α-acetamino-α-carbethoxy-β-(3-indole)-propionate.The HPLC system consisted of Hypersill BDS C18 column(4.6 mm×200 mm,5um),methanol-water(60:40 v/v) mixture as mobile phase,detection wavelength 233nm,flow rate 1.0 ml.min-1 and column temperature at room temperature.The linear range is 4～20 μg(r=0.9999),and RSD less than 0.68%,indicating that the method was simple,quick,accurate,and suitable for the quality control of the product α-acetamino-α-carbethoxy-...

Key words：HPLC; α-acetamino-α-carbethoxy-β-（3-indole）-propionate.; analysis

收稿日期：2005-09-08

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