| 第 36 卷第 4 期 |  | Vol. 36 No. 4 | | 2006 年 8 月 | Aug 2006 |
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所属栏目：分析与测试
高效液相色谱法测定甲氧苄啶氯化钠注射液中甲氧苄啶的含量 |
| 谢燕; 陈明; 肖艳萍(湖南医药工业研究所; 湖南化工研究院测试中心; 湖南长沙) |
| 摘 要：建立了甲氧苄啶氯化钠注射液中甲氧苄啶含量的HPLC测定方法。本法采用C18色谱柱,流动相为0.1mol/L磷酸二氢钾溶液(用磷酸调节pH值至2.5)-甲醇的混合溶液(按v∶v=70∶30配制),检测波长为280nm,流速为1.0mL/min。实验数据表明:甲氧苄啶在0.38～1.37μg的范围内,峰面积与进样量呈良好线性关系(r=0.9995),回收率RSD=0.69%,最低检测限为1ng,分析结果准确,可用于测定甲氧苄啶氯化钠注射液中甲氧苄啶含量。 |
| 关键词：甲氧苄啶氯化钠注射液; 高效液相色谱法; 甲氧苄啶 |
| 中图分类号：O621.3 文献标识码：A 文章编号：1009-9212(2006)04-0064-03 |
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| Quantification of Trimethoprim in Trimethoprim and Sodium Chloride Injection Solution by HPLC |
| XIE Yan1; CHEN Ming2; XIAO Yan-ping1 （1. Hunan Institute of Pharmaceutical Industry; Changsha 410007; China; 2 . Testing Center of Hunan Research Institute of Chemical Industry; China） |
| Abstract：A HPLC method for trimethoprim in trimethoprim and sodium chloride injection solution was establish. The HPLC condition consisted of C18 column(250 mm×4.6 mm,5 μm),mobile phase of 0.1 mol/L monopotassium phosphate-methanol(70 : 30,v/v) at the flow rate of 1.0 mL/min and at detection wavelength 280 nm. The calibrattion linear curve of trimethoprim was in the range of 0.38～1.37 μg(r=0.999 5)with RSD of 0.69%,and the detection limit was 1 ng. This method was very sensitive and reliable,and could be used to mea... |
| Key words：trimethoprim and sodium chloride injection solution; HPLC; trimethoprim |
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收稿日期：2006-06-22
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