| 第 36 卷第 5 期 |  | Vol. 36 No. 5 | | 2006 年 10 月 | Oct 2006 |
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所属栏目：分析与测试
高效液相色谱法测定阔草清中间体含量的方法学研究 |
| 楚刚辉; 程靖(华中师范大学农药与化学生物学教育部重点实验室; 湖北武汉; 新疆喀什师院生命与环境科学系; 新疆喀什) |
| 摘 要：建立了HPLC法测定阔草清中间体N-(2,6-二氟苯基)-5-氨基-1,2,4-三唑-3-磺酰胺含量的方法:色谱柱Zorbax Eclipse XDB-C18(4.6 mm×150 mm,5μm);以v(甲醇)∶v(水)=25∶75(水:25 mmol/L Na2HPO4-H3PO4,pH=7.8)作为流动相;检测波长为214 nm,流速0.6 mL/min,柱温35℃。结果表明阔草清中间体在0.019 9～0.2991μg内呈良好线性关系,r=0.9999。该方法柱效高、灵敏、快速、准确,可用于阔草清中间体含量的测定。 |
| 关键词：高效液相色谱法; 阔草清中间体; N-（2; 6-二氟苯基）-5-氨基-1; 2; 4-三唑-3-磺酰胺 |
| 中图分类号：S482.4 文献标识码：A 文章编号：1009-9212(2006)05-0073-03 |
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| Determination of Content of Flumetsulam Intermediate by HPLC |
| CHU Gang-hui1,2;CHENG Jing1(1.Central China Normal University;Key Laboratory of Pesticide and Chemical Biology of Ministry of Education;Wuhan 430079;China;2.KaShi Teachers College;Department of Life and Environmental Science;KaShi 844000;China) |
| Abstract：A HPLC method for determining the content of flumetsulam intermediate N-(2,6-difluorophenyl)-5-amino-1,2,4-triazole-3-sulfonamide was established.The HPLC system was as follow:inertsil Zorbax Eclipse XDB-C18 column(4.6 mm×150 mm,5 μm),v(methyl alcohol)∶v(water)=25∶75(water:25 mmol/L disodium hydrogen phosphate-phosphoric acid,pH=7.8)as mobile phase at flow rate of 0.6 mL/min,UV detection at 214 nm and the column temperature of 35℃.Result showed that the N-(2,6-difluorophenyl)-5-amino-1,2,4-triazole-3-sulfon... |
| Key words：HPLC; flumetsulam intermediate; N-（2; 6-difluorophenyl）-5-amino-1; 2; 4-triazole-3-Sulfonamide |
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收稿日期：2006-06-15
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