| 第 36 卷第 5 期 |  | Vol. 36 No. 5 | | 2006 年 10 月 | Oct 2006 |
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所属栏目：分析与测试
HPLC法测定芦竹碱 |
| 丁玲玲; 王守凯; 臧娜(中钢集团鞍山热能研究院; 辽宁鞍山) |
| 摘 要：采用Hypersill BDS C18色谱柱,以磷酸二氢钠缓冲液/甲醇(70:30,v/v)为流动相,流速0.8 mL/min,275 nm为检测波长,pH=2.4～2.5,建立测定芦竹碱的高效液相色谱法。进样量1～13μg的范围内线性关系良好(r=0.9998)。RSD不大于0.66%。回收率98%～102%,方法操作简单,分析速度快、准确,适用于芦竹碱的产品质量控制分析。 |
| 关键词：芦竹碱; HPLC; 分析 |
| 中图分类号：O629.7 文献标识码：A 文章编号：1009-9212(2006)05-0070-03 |
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| Determination of Gramine by HPLC |
| DING Ling-Ling; WANG Shou-kai; ZANG Na（China Steel Group Anshan Research Institute of Thermo-energy; Liaoning Anshan 114044; China） |
| Abstract：A HPLC method was established for the determination of gramine.The HPLC system consisted of Hypersill BDS C18 column(4.6 mm×200 mm,5 μm,buffer solution(NaH2PO4)/methanolmixture(70/30,v/v)as mobile phase,detection wavelength 275 nm,flow rate 0.8 mL·min-1,pH 2.4～2.5,and column temperature at room temperature.The linear range was 1～13 μg(r=0.9998),RSD less than 0.66%,and the average recovery was 98%～102%.The method is simple,quick and accurate,and suitable for the quality control of gramine. |
| Key words：gramine; HPLC; analysis |
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收稿日期：2006-06-26
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