第 36 卷第 6 期 Vol. 36 No. 6 2006 年 12 月 Dec 2006

所属栏目：分析与测试 雷洛昔芬中间体4-甲氧基-α-[(3-甲氧基苯基)硫代]苯乙酮含量的反相高效液相色谱测定

张艳林; 胡德禹; 宋宝安; 卢平; 杨松; 樊玲娥; 刘平生; 薛伟(贵州大学精细化工研究开发中心; 贵州贵阳; 绿色农药与生物工程教育部重点实验室)

摘　要：采用反相高效液相色谱法,以v(甲醇)v∶(水)=802∶0作为流动相,用C18柱和二极管阵列检测器(DAD,282nm),测定4-甲氧基--α[(3-甲氧基苯基)硫代]苯乙酮的含量。结果表明,4-甲氧基--α[(3-甲氧基苯基)硫代]苯乙酮在0.02～0.90μg内呈良好的线性关系,r=0.99999。该方法灵敏、快速、准确,可用于4-甲氧基--α[(3-甲氧基苯基)硫代]苯乙酮含量的测定。

关键词：4-甲氧基-α-[（3-甲氧基苯基）硫代]苯乙酮; 检测; 高效液相色谱

中图分类号：O657.7+2　　文献标识码：A　　文章编号：1009-9212(2006)06-0065-03

Determination of Content of Raloxifen Intermediate 4-Methoxy-α-[(3-methoxyphenyl)thio]Acetophenone by R-HPLC

ZHANG Yan-lin;HU De-yu;SONG Bao-an;LU ping;YANG Song;FAN Ling-e;LIU Ping-sheng;XUE Wei(Center for Research and Development of Fine Chemicals;China;Key Laboratory of Green Pesticide and Bioengineering;Ministry of Education;GuiZhou University)

Abstract：A quantitative analytical method of 4-Methoxy-α-[(3-Methoxyphenyl)thio] acetophenone by R-HPLC was described,using v(methanol) ∶ v(water) = 80 ∶ 20 as mobile phase,using Kromasil C18 column at DAD detector(282 nm).Result showed that 4-methoxy-α-[(3-methoxyphenyl)thio] acetophenone was linear(r = 0.999 99)and had a good linear correlation in the range of 0.02~0.90 μg,This suggested that the method was proved to be sensitive,fast and accurate,and could be used for determining the content of 4-methoxy-α-[(3-me...

Key words：4-methoxy-α-[（3-methoxyphenyl）thio]acetophenone; determination; HPLC

基金项目：贵州省省长基金项目(2005374)

收稿日期：2006-10-23

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